Synthesis, characterization and determination of antimicrobial activities of silver nanoparticles derived from black cabbage (Brassica oleracea L.) leaf waste
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Aim: The interest of nanobiotechnology is the manipulation of materials that occur at ultra-small sizes (1-100 nm). Due to its properties such as stability, size distribution, and surface charge, interest in silver nanoparticles (AgNPs) has been increasing in recent years. In this study, it was aimed to synthesize AgNPs in an environmentally friendly, cheap and simple way using black cabbage (Brassica oleracea L.) (BC) green leaf extract and to determine its activity against pathogenic microorganisms.
Materials and Methods: The black cabbage used in the study was obtained from Erzurum pasinler region. AgNO3 was used for the synthesis. Antibiotics (fluconazole, vancomycin, and colistin) together with Escherichia coli ATCC 25922, Bacillus subtilis ATCC 11774, Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, and Candida albicans microorganisms were used to determine the Minimum Inhibitory Concentration (MIC) of BC-AgNPs. Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-Ray (EDX) Spectroscopy, Fourier Transform Infrared (FT-IR) Spectroscopy and Zeta sizer were used for the structural characterization of the synthesized AgNPs.
Result: SEM and TEM results revealed that the morphological structures of AgNPs were spherical and showed good distribution. The zeta potential of BC-AgNPs was measured as -23.5 mV. MIC values (mg/mL) of AgNPs on the pathogen Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC27833 Escherichia coli ATCC25922, Bacillus subtilis ATCC11774 and Candida albicans were 1.00, 2.00, 0.50, 0.25, 0.50, respectively.
Conclusion: Even at very low concentrations, BC-AgNPs were found to be more effective than conventional antibiotics against pathogenic yeast and bacteria. Due to their antipathogenic effects, it seems possible that nanotechnological products containing AgNPs will be used more widely in many fields such as medical applications and food technology in the near future.